An acid, 2,2-dichloropropionic acid (2,2-DCP) is an active ingredient in herbicide (Dalapon®). Using 2,2-DCP as a model substrate, an alkalotolerant bacterium was successfully isolated from the Blue Lake, Turkey. This bacterium is a potential bioremediation agent of recalcitrant xenobiotic halogenated compounds. This study aimed to prove the efficacy of the alkalotolerance Bacillus megaterium BHS1 in degrading 2,2-DCP as the sole source of carbon. Biolog GEN III system and 16S rRNA analysis were used for the identification of the bacterium. It was discovered that the strain BHS1 is Bacillus m ...Daha fazlası
The aim of this study was to isolate and identify Antarctica microalgae collected through the National Antarctic Science Expedition and to examine the growth responses and biochemical contents of Chlorella variabilis under cold and temperate cultivation conditions in growth medium consisting of various nitrogen concentrations under different lighting cycle parameters. In this study, the Antarctic microalgae Chlorella variabilis YTU.ANTARCTIC.001, was cultivated at different temperatures of 4, 13, 20 and 27 °C in modified BG-11 medium with various concentrations of NaNO3 (0, 0.75, 1.5, 2.5 and ...Daha fazlası
Bermet KIDIRALİYEVA | Nurcamal ÖMÜRZAKOVA | Yılmaz KAYA
Çeltik, Antarktika hariç tüm kıtalarda yetiştirilen dünyanın en değerli gıda ürünleri arasında yer almaktadır. Küresel ölçekte, ekim alanı açısından değerlendirildiğinde buğdaydan sonra ikinci sırada yer almakla birlikte verimi yaklaşık olarak toplam buğday verimine eşittir. Kırgızistan, çeltik yetiştiriciliği bakımından önde gelen ülkeler arasında yer almamakta, bu bölgede sadece yerel ölçekte üretim yapılmaktadır. Kırgızistan’ın güney bölgesi çeltik tarımına elverişli olduğu için son 10 yılda nispeten çeltik ekim alanları ve tüketimi artmıştır. Çeltiğe ilginin artmasına; nüfus artışı, ekonom ...Daha fazlası
This study presents the initial structural model of L-haloacid dehalogenase (DehLBHS1) from Bacillus megaterium BHS1, an alkalotolerant bacterium known for its ability to degrade halogenated environmental pollutants. The model provides insights into the structural features of DehLBHS1 and expands our understanding of the enzymatic mechanisms involved in the degradation of these hazardous pollutants. Key amino acid residues (Arg40, Phe59, Asn118, Asn176, and Trp178) in DehLBHS1 were identified to play critical roles in catalysis and molecular recognition of haloalkanoic acid, essential for effi ...Daha fazlası
The use of molecular markers has become an essential part of molecular genetics through their application in numerous fields, which includes identification of genes associated with targeted traits, operation of backcrossing programs, modern plant breeding, genetic characterization, and marker-assisted selection. Transposable elements are a core component of all eukaryotic genomes, making them suitable as molecular markers. Most of the large plant genomes consist primarily of transposable elements; variations in their abundance contribute to most of the variation in genome size. Retrotransposon ...Daha fazlası
Plants are the sources of many bioactive secondary metabolites which are present in plant organs including leaves, stems, roots, and flowers. Although they provide advantages to the plants in many cases, they are not necessary for metabolisms related to growth, development, and reproduction. They are specific to plant species and are precursor substances, which can be modified for generations of various compounds in different plant species. Secondary metabolites are used in many industries, including dye, food processing and cosmetic industries, and in agricultural control as well as being use ...Daha fazlası
Changing environmental conditions can cause diseases in plants, leading to novel interactions between plants and stress factors. In this respect, the relationships between pathogens and plants regarding epigenetics have still been investigated. Our aim is to examine the effect of leaf blight bacterial causal agent, Pantoea ananatis isolate PaTo34a1, on barley- and rice-specific retrotransposons’ (Nikita, Osr30 and RIRE1, respectively) movements in different rice cultivars (Oryza sativa L. cv. Tosya, Cameo, Arize, Ronaldo, Osmancik-97, Kale, Baldo, Bafra, Hakkari and Vasco) collected from diffe ...Daha fazlası
Tomato is one of the best-studied cultivated dicotyledonous plants in molecular studies. Mobile genetic elements constitute large parts of plant genomes. Retrotransposons are mobile genetic elements within the genome and constitute more than 60 of the tomato genome.Transposable elements (TE) or transposons are DNA sequences that can alter their position within a genome, cause mutations and change the genetic identity of the cells and genome size. We aimed to analyze potato specific-P-Tst-1, P-Tst-3 and P-Tst-6 retrotransposon movements in tomatoes at different developmental stages (mature seed ...Daha fazlası
Mumio is a valuable substance that ranges in color from pale brown to blackish-brown and is obtained from rock layers in various mountain ranges worldwide, particularly in the Tien Shan ranges of Kyrgyzstan. It is also a natural chemical found largely in the Himalayas, created over ages by the progressive degradation of particular plants by bacteria. Mumio is essentially a nutraceutical product with considerable human health advantages. To fully appreciate the predicted impact of mumio use in the medical area, particularly in neurological sciences, more research at both the fundamental biology ...Daha fazlası
There are mounting concerns over the high concentrations of non-biogenic, toxic halogenated organic compounds being liberated into the ecosystem. Therefore, this study’s isolation of a novel bacterium from a contaminated stream in Fatsa, Ordu, Turkey, adept in degrading 2,2-dichloropropionic (of 2,2-DCP) is a welcome endeavor. The ability of the bacterial isolate to utilize 2,2-DCP as the sole carbon and energy source was discovered when the bacterium was observed to grow well on liquid minimal media containing 20 mM of 2,2-DCP, showing a doubling time of 14.2 h. The following genetic and bioc ...Daha fazlası
Due to its economic worth, cotton (Gossypium hirsutum L.) is grown in almost 70 countries and provides income for more than 250 million people. Therefore, producing cotton with having some desired characteristics that includes extended biotic and abiotic stress tolerance, improved fiber quality, promoted nutritional content and increased yield is the main objective for cotton biotechnology. To achieve this goal, many tissue culture and gene transfer techniques are being developed and used throughout the years. As applications for the gene transfer, the Agrobacterium-mediated, particle bombardm ...Daha fazlası
Agrobacterium-mediated transformation of upland rice is established in few numbers of cultivars due to the high cultivar-specificity of regeneration from transformed explants. Further, dehalogenase E (dehE) gene had been characterized in Pseudomonas putida and it produces an enzyme that degrades dalapon. This study aimed to transform Turkish upland rice with the dehE herbicide resistant gene and addresses the challenges of transgenic rice recovery by identifying explant and transformation method. Constructed vector pCAMdehE carrying dehE gene was transferred into the rice shoot apex by Agrobac ...Daha fazlası