Potential of three native Turkish isolates of entomopathogenic nematodes (EPNs) (Steinernema feltiae, S. carpocapsae and Heterorhabditis bacteriophora) was evaluated against Holotrichapion pullum (Gyllenhal) (Coleoptera, Apionidae) under laboratory conditions. Suspensions of EPNs were applied at three concentrations (500, 1000 and 2000 IJs ml-1) and at two temperatures (15 and 20°C). After one week, mortality rate of H. pullum adults was assessed. Mortality rate was higher in the nematode treatments than in the control. All nematodes showed more effect depending on temperature increase. At 20° ...Daha fazlası
The gen us of roo t lesio n nematod es (RLN), Pratylench us, feeds an d reprodu ces in the roo t co rtex of man y plant s pecies. RLN was rep roduced on carrot cu lture by using a pu re cu lture. In vitro tes ts were carried out to determine the effects of plant extracts from three ind igenous plants: h enbane, Hyoscyamus niger; b ead-tree, Melia a zedarah and commo n cocklebu r, Xanth ium strumarium on P. tho rn ei (the major wh eat root-les ion nematode). The nematodes were expos ed for 12, 24, 48, 72 an d 96 hrs in three concen tratio ns of plant extracts. Th e effect of plant extracts was ...Daha fazlası
Background Entomopathogenic fungi are a potential biological control agent for many pests. In this study, 14 native Beauveria bassiana isolates were molecularly identified and assessed for their virulence and mortality against adults of the polyphagous planthopper Orosanga japonica, Melichar (Hemiptera: Ricaniidae) a polyphagous sap-feeding insect, under laboratory conditions. Results Isolates obtained from naturally infected adults O. japonica were molecularly identified as B. bassiana by sequencing the internal transcribed spacer and 26S large subunit of ribosomal DNA. In the bioassay, the r ...Daha fazlası
The efficacy of the two entomopathogenic fungi, Metarhizium anisopliae (TR 106) and Beauveria bassiana (TR 217), was tested against the adults of the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Chrysomelidae: Bruchinae) in laboratory. Two concentrations of conidial suspensions (1 x 10(6) and 1 x 10(8) conidia/ml) of M. anisopliae and B. bassiana isolates were directly applied on four pairs of adult beetles in Petri dishes (2 ml/dish) and then incubated at two different climatic regimes; 22 +/- 1 degrees C and 26 +/- 1 degrees C with 70 +/- 5% RH, 16:8 h light-to-dark. Lethal time v ...Daha fazlası