Background Entomopathogenic fungi are a potential biological control agent for many pests. In this study, 14 native Beauveria bassiana isolates were molecularly identified and assessed for their virulence and mortality against adults of the polyphagous planthopper Orosanga japonica, Melichar (Hemiptera: Ricaniidae) a polyphagous sap-feeding insect, under laboratory conditions. Results Isolates obtained from naturally infected adults O. japonica were molecularly identified as B. bassiana by sequencing the internal transcribed spacer and 26S large subunit of ribosomal DNA. In the bioassay, the r ...Daha fazlası
During June and August in 2018, kiwifruit (cv. Hayward) plants exhibiting symptoms of charcoal rot including chlorosis, necrosis, leaf shedding, root rot, and trunk dry rot were observed with an incidence of up 5?n three commercial orchards located in Altınordu, Fatsa and Perşembe districts of Ordu province. After surface sterilization with 1?aClO, small pieces excised from root tissues of diseased plants were rinsed twice in sterile distilled water, dried on sterile filter papers and aseptically transferred to plates containing potato dextrose agar. Ten isolates from the plates were identifie ...Daha fazlası
In October 2020, the presence of an intense Fusarium dry rot disease was detected during a survey of potato tubers in the production storage facilities in Chui region, Kyrgyzstan. The species of Fusarium isolates, obtained from infected tubers, were identified as Fusarium sambucinum by using morphological features and phylogenetic analyses of the transcription elongation factor 1-alpha (EF1-alpha) gene. The isolates were pathogenic to potato tubers, and Koch
A total of 30 Rhizoctonia isolates were collected from plants with Rhizoctonia-like symptoms in kale growing areas in Ordu province during the 2014-2015 growing seasons. All the isolates were identified using nuclear staining tecnique and the nucleotide sequence analysis of the nuclear rDNA internal transcribed spacer (ITS)1-5.8S-ITS2 region. The most prevalent anastomosis group (AG) was AG 2-1 (36.67?f isolates), followed by AG-A (30? AG 4 HG-I (10? AG 5 (6.67? AG-Fb (6.67? AG-E (6.67?and AG-K (3.33? Cultural characteristics showed that randomly selected RsCB-13 (AG 2-1), RsCB-20 (AG 4 HG-I), ...Daha fazlası
The efficacy of the two entomopathogenic fungi, Metarhizium anisopliae (TR 106) and Beauveria bassiana (TR 217), was tested against the adults of the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Chrysomelidae: Bruchinae) in laboratory. Two concentrations of conidial suspensions (1 x 10(6) and 1 x 10(8) conidia/ml) of M. anisopliae and B. bassiana isolates were directly applied on four pairs of adult beetles in Petri dishes (2 ml/dish) and then incubated at two different climatic regimes; 22 +/- 1 degrees C and 26 +/- 1 degrees C with 70 +/- 5% RH, 16:8 h light-to-dark. Lethal time v ...Daha fazlası