Investigation of the tumor microenvironment, hypoxia and angiogenesis by immunohistochemical and histopathological methods in canine mammary tumors

The tumor microenvironment (TME) is an important component for studying tumor behavior in several cancers in human beings. However, little information regarding the role of the TME in canine mammary tumors (CMTs) is available compared to humans. In this study, the aim was to investigate the relationship between the TME, hypoxia and angiogenesis through CD31, VEGF, HIF-1a, CD68 and CD163 expression by using immunohistochemical (IHC) methods in formalin-fixed paraffin-embedded canine mammary tumor samples [(n34: malignant (n28) and benign (n6)], to compare them with the clinicopathological features of tumors, and to analyze the relationship between them. There was no significant relationship between CD31, VEGF, HIF-1a, CD68 and CD163 expression in malignant tumors compared to benign tumors (P>0.05). There was an association between microvessel density and clinicopathological variables (the tumor size P0.013, the presence of necrosis P0.022) and individual histological grade (G2 vs. G3 P0.028) in malignant tumors. While there was a positive correlation between CD68 and CD163 in malignant tumors in the dogs (P<0.01), no correlation was determined between other antibodies. Immunohistochemical determination of the level of angiogenesis in the TME may give further useful information about the angiogenic potential and grading of the clinical aggressiveness of some CMTs.

Keyword: angiogenesis; canine mammary tumors; tumor-associated macrophages; tumor microenvironment

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tumors between malignant mammary relationship angiogenesis canine benign information compared correlation expression clinicopathological HIF-1a positive presence necrosis individual histological macrophages tumor-associated Keyword aggressiveness clinical grading potential angiogenic useful variables further determination Immunohistochemical antibodies determined density
Tam metin
Detaylı Görünüm
Yayın Adı
(dc.title)
Investigation of the tumor microenvironment, hypoxia and angiogenesis by immunohistochemical and histopathological methods in canine mammary tumors
Yazar/lar
(dc.contributor.yazarlar)
Erdinc Guner, Fatih Hatipoglu
Yayın Türü
(dc.type)
Makale
Dil
(dc.language)
İngilizce
Yayımlanma Yılı
(dc.date.issued)
2023
Ulusal/Uluslararası
(dc.identifier.ulusaluluslararasi)
Uluslararası
Kaynak
(dc.relation.journal)
Veterinarski arhiv
Süreli Sayı
(dc.identifier.issue)
6
Cilt/Sayı
(dc.identifier.volume)
93
Sayfa
(dc.identifier.startpage)
665-682
ISSN/ISBN
(dc.identifier.issn)
ISSN: 0372-5480; Online ISSN: 1331-8055
Yayıncı
(dc.publisher)
University of Zagreb, Croatia
Veri Tabanları
(dc.contributor.veritaban)
Web of Science Core Collection
Veri Tabanları
(dc.contributor.veritaban)
Kaynak web sitesi
Veri Tabanları
(dc.contributor.veritaban)
Scopus
İndex Türü
(dc.identifier.index)
SCI Expanded
İndex Türü
(dc.identifier.index)
Scopus
Etki Faktörü
(dc.identifier.etkifaktoru)
0,5 / 2022-WOS / Son 5 yıl: 0,6
Özet
(dc.description.abstract)
The tumor microenvironment (TME) is an important component for studying tumor behavior in several cancers in human beings. However, little information regarding the role of the TME in canine mammary tumors (CMTs) is available compared to humans. In this study, the aim was to investigate the relationship between the TME, hypoxia and angiogenesis through CD31, VEGF, HIF-1a, CD68 and CD163 expression by using immunohistochemical (IHC) methods in formalin-fixed paraffin-embedded canine mammary tumor samples [(n34: malignant (n28) and benign (n6)], to compare them with the clinicopathological features of tumors, and to analyze the relationship between them. There was no significant relationship between CD31, VEGF, HIF-1a, CD68 and CD163 expression in malignant tumors compared to benign tumors (P>0.05). There was an association between microvessel density and clinicopathological variables (the tumor size P0.013, the presence of necrosis P0.022) and individual histological grade (G2 vs. G3 P0.028) in malignant tumors. While there was a positive correlation between CD68 and CD163 in malignant tumors in the dogs (P<0.01), no correlation was determined between other antibodies. Immunohistochemical determination of the level of angiogenesis in the TME may give further useful information about the angiogenic potential and grading of the clinical aggressiveness of some CMTs.
Özet
(dc.description.abstract)
Keyword: angiogenesis; canine mammary tumors; tumor-associated macrophages; tumor microenvironment
URL
(dc.rights)
http://vetarhiv.vef.unizg.hr/papers/2023-93-6-5.pdf?cimirliilosiscscripkpqj
DOI
(dc.identifier.doi)
10.24099/vet.arhiv.2007
Fakültesi / Enstitütü
(dc.identifier.fakulte)
Veteriner Fakültesi
Bölümü
(dc.identifier.bolum)
Veteriner
Kurumdaki Yazar/lar
(dc.contributor.author)
Fatih HATİPOĞLU
Kayıt No
(dc.identifier.kayitno)
BLBFD8789C
Kayıt Giriş Tarihi
(dc.date.available)
2024-02-06
Not (Yayımlanma Yılı)
(dc.identifier.notyayinyili)
2023
Wos No
(dc.identifier.wos)
WOS:001137174500002
Konu Başlıkları
(dc.subject)
angiogenesis
Konu Başlıkları
(dc.subject)
canine mammary tumors
Konu Başlıkları
(dc.subject)
tumor-associated macrophages
Konu Başlıkları
(dc.subject)
tumor microenvironment
Analizler
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