Immunohistochemical Detection of Tissue Localization of Acute Phase Proteins in Cattle Pneumonic Pasteurellosis

This study was aimed to determine by immunohistochemical methods the expressions of acute phase protein such as Haptoglobin, C-reactive protein and Serum Amyloid in the lungs of cattle suffering from pneumonic pasteurellosis (PP). The material of the study was the lungs of 8-18 month old cattle Holstein with Fibrinous Bronchopneumonia brought for necropsy to the Pathology Department of the Selcuk University Veterinary Faculty between 2019-2020. As a result of microbiological culture from the lungs of these animals, 6 lungs where Pasteurella spp. was isolated were used in the study. Additionally, 6 culture-negative healthy animals obtained from the nearby slaughterhouse were used for lung control. A total of 12 bovine lung samples were subjected to histopathological and immunohistochemical staining. In the histopathological examination, while no microscopic lesions were observed in the lungs of the control group, inflammatory hyperemia and red and grey consolidation stages of fibrinous pneumonia were detected in animals with pneumonic pasteurellosis. Immunohistochemically, immunoreactivity was very mild or without staining for primers in healthy lungs. Animals with pneumonic pasteurellosis showed immunopositivity in haptoglobin (Hp), oedema fluid and inflammatory cell infiltrations (P>0.05). C-reactive protein (CRP) staining showed expression in all criteria (epithelial, capillary, oedema and inflammatory cell infiltration) at all phases, and the highest mean score was found during the red consolidation phase (P<0.01). Serum Amyloid A (SAA) staining showed immunopositive staining in oedema fluid and inflammatory cell infiltrations, and the highest mean scores were during the red and grey consolidation phases (P<0.01). According to the results of the study, it can be considered that acute phase proteins may be important in the development process and/or pathophysiology of the disease, and SAA and CRP may provide more sensitive results for the diagnosis and prognosis of the disease.

Keyword: C-Reactive protein; cattle: holstein; haptoglobin; immunohistochemistry; pneumonic pasteurellosis; serum amyloid A

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Tıklayınız
staining protein pasteurellosis pneumonic inflammatory cattle oedema showed consolidation animals highest histopathological control during results phases haptoglobin immunohistochemical C-reactive infiltrations Amyloid disease healthy criteria (epithelial expression immunopositivity capillary infiltration) Animals primers without pathophysiology amyloid immunohistochemistry
Tam metin
Detaylı Görünüm
Yayın Adı
(dc.title)
Immunohistochemical Detection of Tissue Localization of Acute Phase Proteins in Cattle Pneumonic Pasteurellosis
Yazar/lar
(dc.contributor.yazarlar)
M. Ortatatli, O. Ozdemir, G. Akcakavak, M.B. Ates, F. Hatipoglu
Yayın Türü
(dc.type)
Makale
Dil
(dc.language)
İngilizce
Yayımlanma Yılı
(dc.date.issued)
2023
Ulusal/Uluslararası
(dc.identifier.ulusaluluslararasi)
Uluslararası
Kaynak
(dc.relation.journal)
Israel Journal of Veterinary Medicine
Süreli Sayı
(dc.identifier.issue)
4
Cilt/Sayı
(dc.identifier.volume)
78
Sayfa
(dc.identifier.startpage)
28-38
ISSN/ISBN
(dc.identifier.issn)
ISSN: 0334-9152; Online ISSN: 2304-8859
Yayıncı
(dc.publisher)
The Israel Veterinary Medical Association
Veri Tabanları
(dc.contributor.veritaban)
Web of Science Core Collection
Veri Tabanları
(dc.contributor.veritaban)
Kaynak web sitesi
Veri Tabanları
(dc.contributor.veritaban)
Scopus
İndex Türü
(dc.identifier.index)
SCI Expanded
İndex Türü
(dc.identifier.index)
Scopus
Etki Faktörü
(dc.identifier.etkifaktoru)
0,5 / 2022-WOS / Son 5 yıl: 0,5
Özet
(dc.description.abstract)
This study was aimed to determine by immunohistochemical methods the expressions of acute phase protein such as Haptoglobin, C-reactive protein and Serum Amyloid in the lungs of cattle suffering from pneumonic pasteurellosis (PP). The material of the study was the lungs of 8-18 month old cattle Holstein with Fibrinous Bronchopneumonia brought for necropsy to the Pathology Department of the Selcuk University Veterinary Faculty between 2019-2020. As a result of microbiological culture from the lungs of these animals, 6 lungs where Pasteurella spp. was isolated were used in the study. Additionally, 6 culture-negative healthy animals obtained from the nearby slaughterhouse were used for lung control. A total of 12 bovine lung samples were subjected to histopathological and immunohistochemical staining. In the histopathological examination, while no microscopic lesions were observed in the lungs of the control group, inflammatory hyperemia and red and grey consolidation stages of fibrinous pneumonia were detected in animals with pneumonic pasteurellosis. Immunohistochemically, immunoreactivity was very mild or without staining for primers in healthy lungs. Animals with pneumonic pasteurellosis showed immunopositivity in haptoglobin (Hp), oedema fluid and inflammatory cell infiltrations (P>0.05). C-reactive protein (CRP) staining showed expression in all criteria (epithelial, capillary, oedema and inflammatory cell infiltration) at all phases, and the highest mean score was found during the red consolidation phase (P<0.01). Serum Amyloid A (SAA) staining showed immunopositive staining in oedema fluid and inflammatory cell infiltrations, and the highest mean scores were during the red and grey consolidation phases (P<0.01). According to the results of the study, it can be considered that acute phase proteins may be important in the development process and/or pathophysiology of the disease, and SAA and CRP may provide more sensitive results for the diagnosis and prognosis of the disease.
Özet
(dc.description.abstract)
Keyword: C-Reactive protein; cattle: holstein; haptoglobin; immunohistochemistry; pneumonic pasteurellosis; serum amyloid A
URL
(dc.rights)
http://www.ijvm.org.il/sites/default/files/akcakavak_pages_from_4book__vet_4_december_2023_1-7.pdf
Fakültesi / Enstitütü
(dc.identifier.fakulte)
Veteriner Fakültesi
Bölümü
(dc.identifier.bolum)
Veteriner
Kurumdaki Yazar/lar
(dc.contributor.author)
Fatih HATİPOĞLU
Kayıt No
(dc.identifier.kayitno)
BL6F1E5BDF
Kayıt Giriş Tarihi
(dc.date.available)
2024-02-06
Not (Yayımlanma Yılı)
(dc.identifier.notyayinyili)
2023
Konu Başlıkları
(dc.subject)
C-Reactive protein
Konu Başlıkları
(dc.subject)
cattle: holstein
Konu Başlıkları
(dc.subject)
haptoglobin
Konu Başlıkları
(dc.subject)
immunohistochemistry
Konu Başlıkları
(dc.subject)
pneumonic pasteurellosis
Konu Başlıkları
(dc.subject)
serum amyloid A
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